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KMID : 0545120170270122241
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Journal of Microbiology and Biotechnology
2017 Volume.27 No. 12 p.2241 ~ p.2244
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Heterometal-Coordinated Monomeric Concanavalin A at pH 7.5 from Canavalia ensiformis
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Chung Nam-Jin
Park Yeo-Reum
Lee Dong-Heon
Oh Sun-Young
Park Jung-Hee
Lee Seung-Jae
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Abstract
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The structure of concanavalin A (ConA) has been studied intensively owing to its specific interactions with carbohydrates and its heterometal (Ca2+ and Mn2+) coordination. Most structures from X-ray crystallography have shown ConA as a dimer or tetramer, because the complex formation requires specific crystallization conditions. Here, we reported the monomeric structure of ConA with a resolution of 1.6 A, which revealed that metal coordination could trigger sugar-binding ability. The calcium coordination residue, Asn14, changed the orientation of carbohydrate-binding residues and biophysical details, including structural information, providing valuable clues for the development and application of detection kits using ConA.
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KEYWORD
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Concanavalin A, lectins, heterometal coordination, microbial detection, sugar binding region
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